Structural changes of bacteriophage φ29 upon DNA packaging and release
نویسندگان
چکیده
منابع مشابه
Structural changes of bacteriophage phi29 upon DNA packaging and release.
Cryo-electron microscopy three-dimensional reconstructions have been made of mature and of emptied bacteriophage phi29 particles without making symmetry assumptions. Comparisons of these structures with each other and with the phi29 prohead indicate how conformational changes might initiate successive steps of assembly and infection. The 12 adsorption capable 'appendages' were found to have a s...
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DNA packaging in the bacteriophage phi29 involves a molecular motor with protein and RNA components, including interactions between the viral connector protein and molecules of pRNA, both of which form multimeric complexes. Data are presented to demonstrate the higher order assembly of pRNA together with the affinity of pRNA:pRNA and pRNA:connector interactions, which are used to propose a mode...
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0377 You probably never tried to put toothpaste back into the tube, but if you did, you’d have a good idea of what the Bacillus subtilis bacteriophage φ29 experiences as it crams its DNA into a protein capsid shell following replication. Scientists have speculated that this bacteria-infecting “phage” accomplishes this energy-intensive task by rotating a connector complex at the opening that fee...
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We calculate the forces required to package (or, equivalently, acting to eject) DNA into (from) a bacteriophage capsid, as a function of the loaded (ejected) length, under conditions for which the DNA is either self-repelling or self-attracting. Through computer simulation and analytical theory, we find the loading force to increase more than 10-fold (to tens of piconewtons) during the final th...
متن کاملForces during bacteriophage DNA packaging and ejection.
The conjunction of insights from structural biology, solution biochemistry, genetics, and single-molecule biophysics has provided a renewed impetus for the construction of quantitative models of biological processes. One area that has been a beneficiary of these experimental techniques is the study of viruses. In this article we describe how the insights obtained from such experiments can be ut...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 2006
ISSN: 0261-4189,1460-2075
DOI: 10.1038/sj.emboj.7601386